有機(jī)氯殺蟲劑的提取(液相色譜)

Czech Republic Nanjing Analytical Instrument Division, Inc
Nanjing Branch Czech analytical instruments Co., Ltd. is a professional research, development, production, gas chromatograph, liquid chromatograph, data processor, and HPLC chromatography workstation dedicated spare parts,

consumables of high-tech enterprises. The company has long been engaged in a number of HPLC chromatographic analysis of the research and development and application of senior engineers, professors, in the

chromatographic and spectral types of equipment maintenance, repair, and debug, such as strong technical force.

 "In order to survive the quality, service and development" and "break its promise to once a decade to win the trust to be" business philosophy, under the guidance of our company has won, including the province and outside

the scientific research units, institutions and enterprises, such as quality inspection stations institutions, including the long-term support of many users. Is a gas, liquid chromatography and other various high-precision analytical

instruments sales, maintenance and consumables to provide a variety of analytical instruments for professional firms. Warmly welcome customers to come to consultations, negotiations!
 
 Business Scope:
1, to help you create a room and chromatography chromatography analysis, chromatography operator training.

2, sales of various types of imported, domestic analytical instruments and related accessories, samples, reagents.

3, gas chromatography, HPLC, UV spectrophotometer, atomic absorption spectrophotometer, analytical instruments such as installation and debugging, functional improvements, maintenance repairs.

 4, sample analysis of external detection task to undertake, given the report of the

有機(jī)氯殺蟲劑的提取

試劑：二氯甲烷、丙酮、甲醇、無水硫酸鈉（訂貨號(hào)：ECSS50K）

萃取柱：Enviro-Clean 通用型C18萃取柱（訂貨號(hào)：ECUNIC18）萃取柱的預(yù)處理：將10ml二氯甲烷注入萃取柱，浸潤(rùn)約1.5分鐘后，使其通過柱子流出。

然后向萃取柱中加10ml丙酮，同樣浸潤(rùn)約1.5分鐘，使其通過柱子流出。萃取柱在負(fù)壓下用空氣干燥幾秒鐘。然后向柱中加入約10ml甲醇，浸潤(rùn)約1.5分鐘。從現(xiàn)在起到加入樣品的過程中不可以讓柱變干，使甲醇通過柱子流出，剩余部分以剛好浸沒填料為準(zhǔn)。有機(jī)氯殺蟲劑（固相萃取液相法）       有機(jī)氯殺蟲劑的提取

再加入約20ml去離子水，使大部分水通過柱子流出，以不讓吸附劑變干為準(zhǔn)。
樣品加入：用濃硫酸(大約每升30滴)將樣品的PH值調(diào)到2，不要用PH紙來測(cè)量去離子水的PH值，這樣誤差會(huì)很大。有機(jī)氯殺蟲劑的提取  向樣品加入5ml甲醇(可選)和一些替代物質(zhì)，混勻。在負(fù)壓下把樣品加入柱子頂端，樣品在15-20分鐘通過柱子是理想的，并讓柱子在真空下干燥10分鐘。

提取物洗脫：在柱子下放置接收管或是收集小瓶。在樣品瓶中加入10ml丙酮收集剩余物質(zhì)，然后注入柱中，使溶液浸潤(rùn)一分鐘后，將丙酮抽入收集裝置中，用10ml的二氯甲烷重復(fù)此步驟兩次，將提取物通過無水硫酸鈉干燥。

有機(jī)氯殺蟲劑（固相萃取液相法）       有機(jī)氯殺蟲劑的提取
用二氯甲烷仔細(xì)清洗收集裝置并將溶液加入無水硫酸鈉干燥。
富集和分析：小心地濃縮提取物，必要時(shí)可以更換溶劑。
注意：大部分的提取誤差是由條件比較差的濃縮設(shè)備引起的。Enviro-Clean通用型C18萃取柱可用于標(biāo)準(zhǔn)真空歧管(#VMFF-016GL)、標(biāo)準(zhǔn)固相萃取圓盤(#EUCTVAC6)和轉(zhuǎn)換器（#ECUCTADP）。在干燥和振蕩時(shí)移動(dòng)柱子或是在
柱底開孔引出濕氣都可以加快干燥的速度。干燥次數(shù)應(yīng)適當(dāng)以使柱子不要太干，不然重現(xiàn)性會(huì)變差。
該柱是Horizon科技專門為Horizon SPE DEX4790設(shè)計(jì)制造的。

檢測(cè)儀器：

產(chǎn)品規(guī)格：LC-600

主要配置：

P600寶石恒流泵                                 V600紫外檢測(cè)器         
7725i六通進(jìn)樣閥                               進(jìn)口C18柱  150x4.6 5u  
WS600色譜工作站                             ECD

（也可根據(jù)客戶的需求，由我們的人員進(jìn)行配置）

有機(jī)氯殺蟲劑（固相萃取液相法）     有機(jī)氯殺蟲劑的提取